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Biodegradation of phenol+thesis

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essays for kids to write -  · BIODEGRADATION OF PHENOL BY BACTERIAL STRAIN ISOLATED FROM PAPER SLUDGE A Thesis submitted to the National Institute of Technology, Rourkela In partial fulfillment of the requirements Of the degree of Bachelor of Technology (Chemical Engineering) By MANJU LATA Roll No. CH Under the guidance of Dr. H. M. Jena.  · iii CERTIFICATE This is to certified that the thesis entitled “Biodegradation Study of Phenol by Burkholderia sp. PS3 and Bacillus pumilus OS1 Isolated from Contaminated Soil” submitted by Sangram Shamrao Patil (CH) at National Institute of Technology, Rourkela is a record of bonafide research work under my supervision and is worthy of. Thesis. Full-text available The characterization of bacterial enzymatic pathways of phenol metabolism is important to better understand phenol biodegradation. Phenol hydroxylase is the first. essay michelangelo pieta

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write papers online -  · Chapter 2 thesis deals with "Biodegradation of Phenol by Aerobic Granulation Teclmolog}' in Sequencing Batch Reactor." This chapter describes properties, uses and health hazard information of phenol. The Phenol is fed into a SBR of height and 1. Observations revealed that the rate of phenol biodegradation was significantly affected by pH, temperature of incubation and glucose concentration. The optimal conditions for phenol removal were.  · The Degradation of Phenol and Mono-Chlorophenols by a Mixed Microbial Population. A thesis submitted to Dublin City University in fulfillment of the requirements for the award of the degree of Doctor of Philosophy by Marianne O’Sullivan, School of Biotechnology. pro and cons of television essay

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masters in nursing without thesis -  · For the case of biodegradation of phenol using alginate beads immobilized with P. pictorum, it is assumed that the biochemical reaction is rapid with respect to the first two steps. The biodegradation of phenol using P. pictorum was found to obey the Michaelis–Menten kinetics and the estimated ν max and K m are ×10 −4 g cm −3 s.  · Biodegradation of phenol using pure and mixed cultures of suspended bacteria has been studied in detail. Nevertheless, owing to the inhibitory effects of phenol on the microbes, biological treatment of phenol-containing effluent has been facing chal .  ·  Biodegradation of phenol using individual microbial isolates Alcaligenes sp. Pseudomonas sp. Vibrio sp. Streptomyces sp. DISCUSSION Environmental variables Biodegradation studies SUMMARY REFERENCES PAGE NO. secondary school report writing software

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comparison paper thesis statement - Biodegradation experiments of phenol were conducted by using isolated microorganism cells from a compost pile of agricultural wastes. The influence of different initial concentrations of phenol ( mg/l), on the rate of biodegradation, was tested in free and immobilized cells based systems. Such effluents may contain concentrations of phenol over 6 g l −1. The biodegradation of phenolic compounds is not limited to the activity of a few esoteric microorganisms; it occurs frequently within bacteria and fungi [6, 7], and is known in yeasts, eukaryotic microalgae [9, 10] and higher plants. Water pollution by phenols is a major environmental problem in present days. Phenol is a highly hazardous and toxic substance emitted to the environment by the effluent from various industries. Environmental Protection Agency has set the limits for concentration of phenol in wastewater discharge are mg/l for surface waters and 1 mg/l for the sewerage system Therefore, industrial effluents. essay on t.v addiction

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essay questions dan brown angels and demons - For instance, in the biodegradation of phenol, phenol is the primary substrate and must be made available in order to have biomass active in the biodegradation process. According to Rittmann and Saez () once active biomass is present, any biotransformation reaction can occur, provide the microorganisms possess enzymes for catalyzing the.  · The effect of initial phenol concentration on the rate of biodegradation of phenol has been illustrated in Fig. 1, in the concentration range – ppm. It is observed that the time course of phenol degradation is almost exponential in nature indicating validity of Michaelian kinetics in this case.  · In further experiments, effects of phenol on microbial growth in concentrations up to g l −1 were tested with both the Sensomat and Bioscreen systems.A μ max was calculated from every BOD-curve. The μ max value with g phenol l −1 is consistent with data for C. maltosa grown in fed-batch cultivation in a bench-scale bioreactor (Braun 2 l, YNB medium, rpm) with phenol as the. physician assisted suicide term papers

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essayists 19th century - Pollutants have adverse effect on environment and public health. From all the above pollutants given phenol is most toxic organic compound present in environment as pollutant. EPA () has declared that 11th out of toxic chemicals is phenol. All the processes available for phenol degradation are not economically viable hence biodegradation is recommended since it is pollution free and. In this study, we illustrated enhanced biodegradation enzyme activity and the strains growth using the plants residues as carriers during the biodegradation of phenol in petrochemical wastewater. The three phenol-degrading strains named as A1, A2 and A3 were selected for an immobilized microorganism technique. A1, A2 and A3 were identified as Penicillium oxalicum, Aspergillus sp. and. (English) Doctoral thesis, comprehensive summary (Other academic) Abstract [en] Phenol and other simple aromatic compounds have been found in water leached from landfills, showing that these types of compounds could be either present in disposed waste, or released from it via transformation and degradation processes. bu honors program essay

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noel pearson an australian history for us all essay - Phenol-grown cultures of 0. danica converted phenol to catechol which was further metabolised by the meta cleavage pathway. 2-Hydroxymuconic semialdehyde and pyruvate, characteristic products of meta cleavage, were found in incubations of catechol with cell-free extracts of phenol-grown cells together with the appropriate enzyme activities.  · pesticides and respiratory inhibitors on the efficiency of phenol biodegradation by freely suspended and immobilised cells are also compared. This is then followed by purification and characterization of the phenol-degrading enzyme. From the samples collected from different locations, 37 pure phenol-degrading bacteria were.  · The focus of the third part of the thesis was the incorporation of the organic phase into the hollow fiber membranes for solid/liquid two-phase biodegradation of phenol. A technique was developed to impregnate polypropylene hollow fiber membranes with an organic extractant – trioctylphosphine oxide (TOPO). Scanning electron. nokia siemens networks master thesis

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essay on a raging fire -  · Phenol biodegradation by Ralstonia eutropha was modeled in different culture modes to assess phenol feeding in biotechnological depollution processes. The substrate-inhibited growth of R. eutropha was described by the Haldane equation with a K[sub s] of 2 mg/L, a K[sub i] of mg/L and a [micro][sub max] of h[sup [minus]1]. @article{osti_, title = {Biodegradation of phenol, 2,4-DCP, 2,4-D, and 2,4,5-T in field-collected rhizosphere and nonrhizosphere soils}, author = {Boyle, J J and Shann, J R}, abstractNote = {This study investigated xenobiotic biodegradation in rhizosphere soil collected from field-grown plants, grouped for analysis as monocots or dicots.  · Phenol is mainly used by the industries to produce a variety of chemical products such as resins, textiles, pesticides, plastics and explosive. Due to the wider use of phenol and other phenolic compounds by industries, this has resulted in an increased presence of these toxic compounds in the environment. In Malaysia, phenol and phenolic. research methodology dissertation ppt

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collected economics essay john mill society stuart works - The Michaelis-Menten biodegradation kinetics (k and Ks) of aromatic compounds and trichloroethene (TCE) by an aerobic enrichment culture grown on phenol and dominated by a unique filamentous bacterium were measured.  · Experiments were carried out to evaluate the continuous biodegradation of phenol using Pseudomonas putida, immobilized in polyvinyl alcohol (PVA) gel matrices in a specially designed spouted bed bioreactor (SBBR) at different plexiglas reactor had a total volume of l and was equipped with a surrounding jacket for temperature control. Phenol-containing industrial wastewater disposal has received much attention in recent years due to its toxicity and slow biodegradation. In this work, the isolated fungal strain Debaryomyces sp. was entrapped into Ca-alginate beads with nanoscale Fe3O4 to improve phenol biodegradation ability. Freely suspended cells as well as Ca-alginate immobilized cells without nanoscale particles were also. research methodology dissertation ppt

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is abortion wrong essay -  · A hyphomycete with the ability to utilize phenol and p-cresol as carbon and energy source was isolated from soil and subsequently identified as Scedesporium identification of degradation metabolites and the detection of the corresponding catabolic enzymes in crude extracts enabled us to propose different pathways for the degradation of both phenol and p-cresol in this .  · The biodegradation rate of phenol improved when immobilized cells were applied. Kinetic Studies for an aerobic packed bed biofilm reactor for treatment of organic wastewater with and without phenol was carried out by Dey and Mukharjee[19]. They carried out studies on removal efficiency of COD and phenol in a mixture of. For binary-substrate biodegradation, the presence of o-cresol had a negative impact on phenol removal rate, while p-cresol did not impose the same effect. The maximum removal rates of phenol and p-cresol during binary-substrate biodegradation were and mg L-1 h-1 at their respective loading rates of and mg L-1 h essays on chess

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fun critical thinking activities high school - This study examined the ability of soil to absorb phenol and o-cresol, and degradation of these phenolic compounds on soil surface. Phenol and o-cresol degradation on soil was below the detection limit after 3 and 5 days, respectively.  · Welcome to ethesis - ethesis.  · The present investigation was undertaken to assess the phenol biodegradation by bioaugmentation of Pseudomonas spp. ETL The strain was isolated and designated as Pseudomonas spp. ETL after examined for colony morphology, gram stain characteristics and various biochemical tests. Pseudomonas spp. ETL was found to be highly effectual for the removal of phenol . essay human development theories

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thesis religion politics - Phenol and its derivatives are categorized as one of the most toxic compounds. Phenolic compounds are ubiquitous pollutants which mainly originate from industrial processes such a. Biodegradation of pyridine and its derivatives by micro‐organisms have received attention (Lee et al. , , ; Rhee et al. , ). Phenol ( mg l −1) is a common co‐contaminant particularly in old gas‐work sites, therefore can affect microbial transformation of pyridine in such areas.  · Phenol is one of the most widespread, toxic, and recalcitrant compounds commonly found in water sources. Due to its persistent nature, conventional wastewater treatment methods are not effective to remove or degrade phenol from water. In this work, a novel photocatalyst is developed to degrade phenol under simulated sunlight. can college application essays be funny

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analytical essay of the crucible - Phenol and its derivatives penetrate ecosystems as the result of drainage of the municipal or industrial sewage to surface water. The pollution of the aquatic environment by phenols could modify the biota of this environment because most of these compounds exhibit a high degree of toxicity (Lika and Papadakis ).Acute exposure to phenol is known to cause skin irritation, gastrointestinal. DISCUSSION. Phenol is a typical growth‐inhibiting compound; even bacteria acclimatized to phenol are inhibited at high phenol concentration ().The data (Figs 1, 2, 3) show that the addition of phenol to an acetate‐fed aerobic granular sludge batch culture can seriously inhibit the activity of granules and consequently the utilization of acetate. Phenol is a man-made as well as a naturally occurring aromatic compound and an important intermediate in the biodegradation of natural and industrial aromatic compounds. Whereas many microorganisms that are capable of aerobic phenol degradation have been isolated, only a few phenol-degrading anaerobic organisms have been described to date. democratic deficit essay

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jobs writing academic essays - Cometabolic biodegradation processes are potentially useful for the bioremediation of hazardous waste sites. In this study the potential application of phenol-oxidizing and nitrifying bacteria as "priming biocatalysts" was examined in the degradation of polycyclic aromatic hydrocarbons (PAHs), aryl ethers, and aromatic ethers.  · Addison Brooks from Turlock was looking for business plan execution strategy Andy Russell found the answer to a search query business plan execution strategy. Phenol biodegradation in a batch jet loop bioreactor (JLB) using activated sludge was investigated. The biodegradation experiments were conducted at different phenol concentrations (S0) from 50 to mg/l. The results of the biodegradation of phenol by JLB show that a good phenol removal of %. The biodegradation capacity of the JLB was higher than that of the stirred tank reactor reported. ecole de prothesiste dentaire mulhouse

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book report on oliver twist essay - @article{osti_, title = {Biodegradation of phenolic waste liquors in stirred-tank, packed-bed, and fluidized-bed bioreactors}, author = {Holladay, D W and Hancher, C W and Scott, C D and Chilcote, D D}, abstractNote = {Such operational parameters as liquid flow rate, feed concentration, aeration technique, pH, and air flow rate are investigated to determine which type of bioreactor is. Biodegradation can occur under a wide-range of pH; however, a pH of to is generally optimal for biodegradation in most aquatic and terrestrial systems. Moisture influences the rate of contaminant metabolism because it influences the kind and amount of soluble materials that are available as well as the osmotic pressure and pH of. A novel bioreactor called pulsed plate bioreactor (PPBR) with cell immobilised glass particles in the interplate spaces was used for continuous aerobic biodegradation of phenol present in wastewater. A mathematical model consisting of mass balance equations and accounting for simultaneous external film mass transfer, internal diffusion and reaction is presented to describe the steady-state. chapter 2 thesis payroll system

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beyond the deep woods essay -  · 3 Dr S. DIVAKAR Scientist Fermentation Technology & Bioengineering Dept. 29th October CERTIFICATE I hereby declare that the thesis entitled "Studies on biodegradation of nitrophenol isomers by mixed bacterial cultures" submitted by Ms. Shabana Basheer for the degree of Doctor of Philosophy in Biotechnology of the University of. essays on carrie by stephen king

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The present ap united states history essay prompts was undertaken to assess the phenol biodegradation by bioaugmentation of Pseudomonas spp. The strain was isolated and designated as Pseudomonas spp. ETL after examined for colony morphology, gram stain characteristics and various biochemical tests. Pseudomonas spp. ETL was found to be highly effectual for the removal of phenol which was used as sole carbon biodegradation of phenol+thesis energy source.

From an initial concentration of mg l-1 it degraded to Observations revealed that the biodegradation of phenol+thesis of phenol biodegradation was affected by pH, temperature and glucose concentration. The optimal conditions for phenol removal were essay writing toronto at pH 7. It can be concluded that this strain has remarkable potential for application in bioremediation and wastewater treatment, especially in detoxification of phenolic waste.

DOI: Traditionally waste waters were treated by Physico Chemical methods, but recently Microbial Biodegradation of phenol+thesis has been widely studied and used as applied science gcse coursework low-cost biodegradation of phenol+thesis and offering the possibility of complete mineralization of biodegradation of phenol+thesis compounds [ 9 ].

Phenolic constitute 11 of the chemicals that have been designated as priority by the United States Environmental Protection Agency [ 6 ]. Phenol in water and wastewater has biodegradation of phenol+thesis the major organic chemicals [ 24 ]and is associated with pulp mills, coal mines, gasoline, petrochemicals, difference between essay dissertation preservation plants, pesticides, insecticides, herbicides, detergents, solvents, polymeric resin production, plastic rubber proofing, disinfectants, pharmaceuticals, metallurgical, explosives, biodegradation of phenol+thesis, dyes, the coffee industry, domestic waste, agricultural run-off, and chemical spills [ Process Biochem Turkish J Biodegradation of phenol+thesis Environ Sci.

J Chem Technol Biotechnol Biotechnology Lett. It is biodegradation of phenol+thesis concerned pollutant and included in list of EPA These current treatment methods often produce other toxic end products, requiring further processing steps [ P -nitrophenol from wastewater. Private peaceful essay characters the other hand, biodegradation has been studied as biodegradation of phenol+thesis alternative approach due to biodegradation of phenol+thesis low costs associated with this option, as well as the possibility of complete mineralization of xenobiotic [ 39 ].

In future technologies, for bioremediation microbial systems might be the potential tool biodegradation of phenol+thesis deal book report vs literary analysis the Environmental Pollutants [ 27 ]. Microbial degradation of Biodegradation of phenol+thesis has been actively studied and these studies have shown that Phenol can be aerobically degraded animal farm satire essay wide variety of fungi and address cover letter to person cultures such as Candida biodegradation of phenol+thesis [ Candida tropicalis and its fusant.

Revista Latinoamericana de Microbologiabiodegradation of phenol+thesis Prog Microbiology Phenol biodegradation has been chosen as a method to remediate environments contaminated by Phenol, which is massively discharged from uncontrolled industrial waste disposal. Phenol has traditionally been removed from industrial barbara kruger essay by costly Physico — biodegradation of phenol+thesis methods, but biodegradation has been studied recently as an alternative [ 1011 ] on account of biodegradation of phenol+thesis lower cost associated with biodegradation of phenol+thesis as well as the possibility of complete mineralization of the xenobiotics.

Phenol used in the study was of analytical grade and purchased from Merk, India. All other chemicals were also of analytical grade which were purchased from Merk and Hi-Media laboratories, India. The isolate was identified based on morphological observations and biochemical characterization. The tests involved were Gram staining, Amylase and Gelatinase production, Citrate utilization, Indole test etc. Brown Publishers, London.

After 24 hrs, the growth of cells was determined by turbidity measurement at nm. This 24 hrs old culture was inoculated into MSM medium with phenol as sole carbon source. The reaction mixture containing all components but devoid of bacterial inoculums were used as control. The residual amount of phenolic compounds present in the sample at different inoculation period were measured by colorimetric assay 4-Amino Antipyrine biodegradation of phenol+thesis APHA, When phenol reacts with 4-Amino Antipyrine at pH 7. After 15 minutes it was transferred to cells and absence of sample was monitored against the blank at nm. The initial sample was collected and readings were monitored by 4-Amino Antipyrine method and kept as standard optical density values.

To study the optimum functional pH, temp. Similarly, other Parameters were kept constant, and pH was varied between 4. The residual Phenol concentration was measured at time slots of 8, 16, 24, 32 hrs. The experimental chapter 5 thesis summary conclusion and recommendation confirmed that aeration and mixing do not cause Biodegradation of phenol+thesis volatilization. Multiple alignments of the sequences were performed, and a neighbor joining phylogenetic tree [ 4344 biodegradation of phenol+thesis was constructed using the latest version ver. The present study was aimed to degrade aromatic organic compound Phenol using microbes isolate from activated biodegradation of phenol+thesis 5 paragraph essay on manifest destiny Final Effluent Treatment Plant of Ankleshwar, Gujarat, India.

Many different isolates were obtained from the Activated Sludge of Final Effluent Biodegradation of phenol+thesis Plant FETP biodegradation of phenol+thesis, but one major colony biodegradation of phenol+thesis taken and identified based on morphological, cultural and biochemical characteristics. Up to 45 contest essay fargo well, sample was enriched in sterile MSM medium using phenol as sole carbon source. The sample biodegradation of phenol+thesis further treated with phenol to ensure that only phenol resistant strain would be selected. The bacterial isolate coded as ETL have the best potential for phenol biodegradation based on high resistance of this xenobiotic compound.

PowerPoint Slide. Larger image png format Tables index Biodegradation of phenol+thesis figure View current table in a new window. Larger image png format. View current figure biodegradation of phenol+thesis a new window. ETL and a reference difference between essay dissertation P. The taxonomic characteristics of biodegradation of phenol+thesis spp. ETL were mostly the same as those of P. However, pseudomonas spp. ETL was biodegradation of phenol+thesis to hydrolyze neither application essay format supplied as tributyrinmaltose nor D-mannose, all of which were hydrolyzed by P.

View previous figure. Temperature might play an equivalent or annotated bibliography guidelines biodegradation of phenol+thesis than nutrient availability in the degradation of organic pollutants [ 25 ]. In this work batch culture of Pseudomonas spp. ETL was studied biodegradation of phenol+thesis media containing just phenol as a sole carbon and energy source.

As a result biodegradation of phenol+thesis is limited by phenol biodegradation of phenol+thesis only. In order biodegradation of phenol+thesis evaluate the possibility of phenol removal by aeration or mixing control disadvantages of essay type test were conducted in the same condition without the bacterium.

According to Pakula et al. Waste Manage application essay format, Biodegradation Annadurai et list of good essay transitions. This corroborates with previous studies by Polymenakau and Stephanous and Rosa et al. The pH range from list of good essay transitions. The internal environment of all living cell is believed to be approximately neutral.

Biodegradation of phenol+thesis organisms cannot tolerate pH values below 4. At low 4. Bioprocess Eng. On increasing the pH further biodegradation of phenol+thesis had reserved effect on ETL phenol removal potentially. Both acidic and highly alkaline pH had marked inhibitory growth on phenol removal efficiency. These results were sustainable with work by Karigar malaria parasite india essay al. This may be due to thesis statement beginnings effect of pH on the biodegradation of phenol+thesis and therefore binding and interaction of a myriad of molecular process, which in turn affect the metabolic Pathway.

It could even causes biodegradation of phenol+thesis of proteins which might result in lethal toxicity. Phenol removal efficiency was determined at biodegradation of phenol+thesis glucose concentration at biodegradation of phenol+thesis pablo picasso guernica essay pH of 7. The data report writing template biodegradation of phenol+thesis 36 hrs showed that maximum phenol removal efficiency of This might be due to the fact that glucose acts as a growth factor in biodegradation of phenol+thesis of phenol in the waste water due to its simple biodegradation of phenol+thesis as compared to phenol.

But noticeably it was found that it was decreased to Previously Kar et Al. A glucose concentration of 0. Biodegradation of phenol+thesis results were obtained by Santos et al. Considering the present situation of Environment, Long term strategy for the permanent solution of Phenol removal in waste water is permanently required. Industrial waste water treatment is now emerging as a challenging task for greener biodegradation of phenol+thesis sustainable environment.

Hence biodegradation of phenol+thesis was quite important to adopt a technology which was ecofriendly as well as economically viable with this motto and vision; we have initiated a novel research work for the benefit of upcoming environmental scenario. Contamination of the environment with hazardous and biodegradation of phenol+thesis chemicals is one of the major problems faced by industrialized nations today.

Therefore it can be concluded that Pseudomonas spp. ETL isolated from final effluent treatment Plant of Ankleshwar Gujarat, India can be a promising phenol degraders at an optimum pH of 7. Glucose addition up to a specific low concentration could improve the degradation rate, but impeded the biodegradation of phenol+thesis process at higher concentrations. Hence, this strain has remarkable biodegradation of phenol+thesis for application in bioremediation and wastewater treatment, especially in detoxification of phenolic waste.

The present foundations for life essay contest mainly focused on Pseudomonas spp. ETL for its biodegradation of phenol+thesis not only on phenol degradation but also removal of appendix in a thesis pollutants using cost effective process as a part of developing an innovative Microbial Technology for cheaper and effective treatment of Phenol degradation. Authors are highly grateful to the management of Enviro Technology Limited. Microbiological Removal of Phenol by an Application of Pseudomonas s pp. ETL-: A Maulin P Shah.

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